Gamma-ray treatment was used to exterminate nematodes, which were harmful to agriculture. When gamma-ray at the dose range of 5~75 kGy was irrdaidated, nematodes were reduced by 40~50%. When irradiated over 30 kGy, almost nematodes were non-mobile and fluorochromatic reactions showed that 60~80% of nematodes with non-mobility died after irradiation. The results of electron spin resonance spectroscopy suggested that radicals generated by radiolysis could damage to nematodes.