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Chemical interaction of tomato and potato tuber moth mediated by leaf volatiles

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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

The potato tuber moth (PTM) is an oligophagous pest on solanaceous crops. We tested adult olfactory behavior and larval development on 5 cultivated tomato varieties leaves including Moneymaker, Campari, Ailsa craig, LA3475 and E6203, and one wild species, S. pimpinellifolium. Bioassay on PTM larvae showed highest developmental performances in Ailsa craig, and lowest in LA3475. Tomato leaf hydro-distilled oils of Moneymaker, Campari, Ailsa craig, S. pimpinellifolium and E6203 showed strong attractiveness and LA3475 exhibited repulsiveness for mated adult PTM of both sexes in two-armed bioassay. The hydro-distilled oils of all tomato leaves contain the fatty acid n-hexadecanoic acid as a major compound. We conformed presence of n-hexandecanoic acid (palmitic acid) in the leaves by GC-MS. LA3475 consists of (1-hexyltetraecyl) cyclohexane (10.5%) as the second major compound which was absent in all other tomato varieties. A standard sample palmitic acid was tested against the pest at 0.1, 1 and 10 mg/ml did not show any significant olfactometer effect. On other hand, phytol showed a weak attractiveness (60.7-63.6%) and (57.6-60.5%) for male and female PTM, respectively at 0.1, 1 and 10 mg/ml. Additionally, SEM of these tomato varieties leaves showed they have trichomes with distinct shapes. We are working on HPLC, and GC-MS analysis of specific chemicals in trichomes of these tomato varieties leaves.

저자
  • Aman Dekebo(Agricultural Science and Technology Institute Andong National University, Republic of Korea, Applied Entomology Program, Department of Bioresource Sciences, Graduate School. Andong National University, Republic of Korea, Department of Plant Medicine, Andong National University, Program of Applied Chemistry, Adama Science and Technology University)
  • Chuleui Jung(Agricultural Science and Technology Institute Andong National University, Republic of Korea, Applied Entomology Program, Department of Bioresource Sciences, Graduate School. Andong National University, Republic of Korea, Department of Plant Medicine, Andong National University)
  • Sunil Aryal(Applied Entomology Program, Department of Bioresource Sciences, Graduate School. Andong National University, Republic of Korea, Entomology Division, Nepal Agricultural Research Council, Khumaltar, Lalitpur)