논문 상세보기
Morphological and Genetic Characterization of Caffeine-Rich and -Poor Tea Tree (Camellia sinensis L.) KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/364244
pp.1-8
In this study, 160 tea tree (Camellia sinensis L.) lines were classified by caffeine content using colorimetric methods. Among them, caffeine-rich lines (HR-78, HR-137, HR-82 and HR-123) and poor lines (HP-85, HP-88, HP-19, and HP-131) were selected. To know the difference in morphological and genetic characters between caffeine-rich and poor lines, we used leaf/shoot growth and RAPD methods. Cluster pattern of morphological characters (leaf width, leaf length, leaf area and shoot length) showed that shoot length was longer in caffein-rich lines than in -poor lines. In genetic analysis, amplified DNA bands having various sizes were detected in RAPD analysis where 30 random primers were used. However, the discriminated primer set that distinguish caffein-rich tree line from -poor lines was not found. These results can be used as the basic data to determine the morphological and genetic differences among caffein-rich and -poor lines
I. INTRODUCTION
II. MATERIALS AND METHODS
2.1 Plant Materials
2.3 Data analysis of leaf morphology
2.4 Genomic DNA isolation and polymerase chainreaction
2.5 Data analysis of polymorphism
III. RESULTS AND DISCUSSION
3.1 Characterization of leaf morphology in selectedtree lines
3.2 Genetic characterization in selected tree lines
IV. Acknowledgements
≫ Literature cited
