Alcohol intake is known to affect various organs in the human body, causing reduction of salivation in the oral cavity. Hypo-salivation effect of alcohol is a common feature, but the mechanism in salivary glands is still poorly studied. Therefore, in this study, the changes in salivary secretion and water channel protein (aquaporin5, AQP5) in salivary glands of mice were investigated after ethanol administration. Animals were divided in to 4 groups with the control, 4 g/kg ethanol, 8 g/kg ethanol and 16 g/kg ethanol administration groups. One hour after ethanol administration, saliva was collected from the oral cavity, and the animals were killed and parotid and submandibular glands were extracted to analyze the histopathology, AQP5 immunihistochemistry and AQP5 protein level. According to the results, the salivation rate decreased irrespective of the ethanol dose in mice, and viscosities increased with increase in ethanol dose. However, there were no pathological changes in parotid and submandibular glands due to ethanol administration. Expression of AQP5 in parotid and submandibular glands decreased with increase ethanol administration These results indicate that the reduction of salivary secretion due to acute alcohol intake is closely related to decrease of the water channel protein such as AQP5 in parotid glands and submandibular glands, rather than the damage of salivary glands.

Introduction
 Materials and Methods
  실험동물 (Experimental animals)
  알코올의 처리 (Ethanol treatment)
  타액수집 (Saliva harvest)
  조직 처리 (Tissue process)
  침샘조직 분석방법 (Tissue analysis)
  자료분석 및 통계처리
 Result
  타액의 유속 및 점조도
  Hematoxylin & Eosin staining (H&E staining )
  AQP5 면역조직화학 및 단백질 발현 변화
 Discussion
 References

• You Sun Lim(Department of Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University)
• Ki-Yeon Yoo(Department of Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University) Correspondence to