Soybean cyst nematode, Heterodera glycines is one of the major plant parasitic nematodes in soybean. Recently, another soybean cyst nematode, H. sojae was recorded from Korea. This study was conducted to determine the geographical distribution of the two soybean cyst nematodes in Gangwon-do and Chungcheongbuk-do, Korea. In Gangwon-do, H. glycines and H. sojae were detected in 12 and 5 of the 53 sites sampled, respectively, while in Chungcheongbuk-do, H. glycines was isolated from 14 of the 46 sites sampled. H. sojae was detected at only one site. Additionally, the two soybean cyst nematodes co-existed in 3 of the 46 sites.