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Evaluation of Pharmacological Activities of Ethanol Extracts Prepared from Selected Korean Medicinal Plants KCI 등재

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한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)
한국식품위생안전성학회 (Korean Society of Food Hygiene and Safety)
초록

In this study, 23 ethanolic extracts from 20 medicinal plants were evaluated for biological activities. Results revealed that of 23 samples, seven samples have demonstrated good antimicrobial activity. Minimum inhibitory concentrations were 0.4-2.0 mg/mL, while minimum bactericidal concentrations were mostly high 0.8- 2.0 mg/mL for selected seven samples. Five samples revealed > 70 mg gallic acid equivalent (GAE)/g of total phenolic contents. Among test samples, six samples exhibited > 80% inhibition of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and only two samples exhibited > 80% inhibition of 2,2’-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals. A total of five test samples revealed Trolox equivalent antioxidant capacity more than 1000 μm/ g. The MTT assay indicated that eight test samples exhibited > 90% viability of murine macrophage cells (RAW 264.7) at 250 μg/mL and suppressed iNOS mRNA expression at transcriptional level when stimulated by lipopolysaccharide (LPS). Some medicinal plants revealed promising results, and so they have prospective for further more inclusive studies.

목차
ABSTRACT
 Materials and Methods
  Plant collection and extraction
  Antimicrobial activity
  Determination of total phenolic content (TPC)
  DPPH radical scavenging activity assay
  ABTS•+ radical scavenging activity
  Trolox equivalent antioxidant capacity (TEAC)
  Cell viability assay
  Anti-inflammatory assay
  iNOS mRNA expression
 Results and Discussion
  Antimicrobial activity
  Total phenolic contents
  DPPH-free radical scavenging assay
  ABTS•+ radical scavenging activity
  Trolox equivalent antioxidant capacity
  Cell viability assay
  Anti-inflammatory assay
  iNOS mRNA expression
 Conclusion
 Reference
저자
  • Deog-Hwan Oh(Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University) Correspondence to
  • IM Zi Eum(Forest Resources Development Institute of Gyeongsangbuk-do)
  • Imran Khan(Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University)