가공식품에서 폴리아크릴산나트륨에 대한 분석 방법을 size-exclusion chromatography를 사용하여 개발하고 유통중인 가공식품에 대해 모니터링 하였다. 분석조건에 대하여 GF-7M HQ column과 UV / VIS 검출기를 피크 모양과 선형성을 기준으로 선택하였으며, 유속, column oven 온도 및 이동상은 각각 0.6 mL/min, 45℃ 및 50 mM sodium phosphate buffer (pH 9.0)으로 선정되었다. 시료의 전처리를 위하여 pH 7.0의 50 mM sodium phosphate buffer로 20℃, 150 rpm에서 3 시간 동안 추출하였다. 확립된 분석법을 검증한 결과 적절한 선택성을 나타내었으며, 검량선은 50~500 mg/L의 범위에서 선정되었고, 이때 검량선의 상관계수(R2)는 0.9985로 나타났다. 폴리아크릴산나트륨의 검출한계(LOD)는 10.95 mg/kg으로 정량한계(LOQ)는 33.19mg/kg의 값을 얻었으며, 정밀도는 intra-day의 경우 3.0~8.3%를, inter day의 경우 1.3~2.6%로 확인되었다. 정확도는 intra-day의 경우 99.6~127.6%를 나타내었으며, interday의 경우 94.3~121.9%로 확인되었다. 유통 중인 가공식품 125품목을 대상으로 폴리아크릴산나트륨의 함유량을 분석한 결과 40품목에서 검출되었으며, 검출량은 식품첨가물공전의 규격인 0.2% 미만으로 검출되었다. 본 연구 결과는 size-exclusion chromatography에 의한 분석방법이 가공식품에서 폴리아크릴산나트륨을 분석하는데 적용할 수 있는 적합한 방법이라는 것을 나타내었다.

An analytical method of sodium polyacrylate in processed food products was developed and monitored by using size-exclusion chromatography. GF-7M HQ column and UV/VIS detector were selected based on peak shape and linearity. Flow rate, column oven temperature, and mobile phase were selected as 0.6 mL/min, 45℃, and 50 mM sodium phosphate buffer of pH 9.0, respectively. Samples for analysis of sodium polyacrylate were extracted with 50 mM sodium phosphate buffer of pH 7.0 for 3 hr at 20℃ and 150 rpm. Analytical method validation revealed proper selectivity and calibration curve was selected in the range of 50-500 mg/L, and correlation coefficient of calibration curve was more than 0.9985. Limit of detection of sodium polyacrylate was 10.95 mg/kg and limit of quantification was 33.19 mg/kg. Accuracy and coefficient of variation for sodium polyacrylate analysis was 99.6-127.6%, 3.0-8.3% for intra-day and 94.3-121.9%, 1.3-2.6% for inter-day, respectively. Sodium polyacrylate was detected in 40 samples among monitored 125 processed food products. Detected contents were less than 0.2%, limited by the Food Additives Code. Results suggest the established size-exclusion chromatography method could be used to analyze sodium polyacrylate in processed food products.

ABSTRACT
 Materials and Methods
  시약
  장비
  Column 및 분석법 확립
  시료 전처리법 확립
  분석법 검증
  표준용액의 제조
  가공식품 시료 제조
  통계처리
 Results and Discussion
  표준물질 용매 선정
  HPLC를 이용한 폴리아크릴산나트륨 분석조건 확립
  시료 전처리 및 추출
  폴리아크릴산나트륨 분석법 검증
  가공식품 중 폴리아크릴산나트륨 함량 분석
 국문요약
 References

• 정은정(전북대학교 식품공학과) | Eun-Jeong Jeong (Department of Food Science & Technology, Chonbuk National University)
• 최유정(전북대학교 식품공학과) | Yoo-Jeong Choi (Department of Food Science & Technology, Chonbuk National University)
• 이근영(식품의약품안전평가원 첨가물포장과) | Gunyoung Lee (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation)
• 윤상순(식품의약품안전평가원 첨가물포장과) | Sang Soon Yun (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation)
• 임호수(식품의약품안전평가원 첨가물포장과) | Ho Soo Lim (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation)
• 김미경(식품의약품안전평가원 첨가물포장과) | MeeKyung Kim (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation)
• 김용석(전북대학교 식품공학과) | Yong-Suk Kim (Department of Food Science & Technology, Chonbuk National University) Correspondence to