The two-spotted spider mite, Tetranychus urticae, is a worldwide agricultural pest that invades a wide range of host crops and rapidly develops resistance to pesticides. T. urticae can be resistant to one acaricide or exhibit multiple resistances or cross resistance to various other acaricides. Acequinocyl inhibits respiration in mitochondria at the ubiquinol oxidation site (Q0) of Complex III of the electron transfer chain. Pyridaben is a METI acaricide that inhibits mitochondrial electron transport at complex I. In this study, we investigated the cross resistance to seven acaricides in acequinocyl- and pyridaben-resistant strain. Furthermore, the frequencies of the I256V and N321S mutations in mitochondrial cytochrome b (cytb) of pyridaben-resistant and fieldcollected strain was analyzed.

• Sung-il Kim(Department of Plant Medicine, Chungbuk National University)
• Hyun Kyung Kim(Department of Plant Medicine, Chungbuk National University)
• Hyun-Na Koo(Department of Plant Medicine, Chungbuk National University)
• Gil-Hah Kim(Department of Plant Medicine, Chungbuk National University)