For the studying of effects of matrine and azadirachtine against Aphidius colemani, it was conducted to test the toxic effect of ecofriendly materials. Matrine and azadirachtine are active ingredient of the most commonly used eco-friendly insecticidal materials against insect pests. The effects of materials are investigated by spraying solution to mummies and adults in laborartory condition (Temp: 25℃; 16L:8D; RH: 50-60%). Each materials was diluted and treated with 5 concentrations, 10 wasps for each replications and 3 replications for each concentration. Fitness of A. colemani was assessed by measuring survival rate, emergence rate, longevity and fecundity. Wasps adults were used for experiments within 48 hours after their hatching. Emergence rates were checked for 12 days after spray to mummies of A. colemani, and survival rates were checked at 24 and 48 hours after vial test for adult parasitoids with 10% sugar solution. Adult wasps were exposed to dry residue of material for 24 hours. Longevity was monitored every 12 hours and fecundity was measured by the number of mummies produced by A. colemani adults.