Bursaphelenchus xylophilus, commonly known as pine wood nematode (PWN) and pathogen of pine wilt disease, is transmitted to new host trees through Monochamus spp.. In this study, five points were set 1 km apart placing the interface of the damaged area in the middle to measure density of the beetles and confirm the presence of PWN in Chungcheongbuk-do, Republic of Korea. The vectors collected from five traps on each point were sorted on weekly basis until the 12th week. We confirmed the presence of PWN in each collected vector by PCR with species-specific ITS primers.

• Donghun Ohn(Division of Forest Diseases and Insect Pest, National Institute of Forest Science)
• Changuk Han(Division of Forest Diseases and Insect Pest, National Institute of Forest Science)
• Ung Gyu Lee(Division of Forest Diseases and Insect Pest, National Institute of Forest Science)
• Chansik Jung(Division of Forest Diseases and Insect Pest, National Institute of Forest Science)