In order to identify the specific antigens for pine wood nematode (PWN), we confirmed that one of the genes commonly found in the transcriptome, proteome and secretory proteins of PWN belonged to the Aldose Reductase (AR) family protein. 36.5 kDa PWN-AR1 was expressed and purified using Baculovirus Expression System. Total 1,546 hybridoma fusion library was generated and screened for specificity to PWN-AR1 by Enzyme Linked Immunosorbent assay (ELISA). Nine clones showed strong immunoreactivity to PWN-AR1 were limited-diluted. Total 864 limited-diluted clones were further screened using PWN-AR1 by ELISA and 34 monoclonal antibody (Mab) clones were selected. 34 Mab clones were further screened using PWN extracts and a standard PWN-infected pine tree extract by ELISA. Finally nine clones were selected and their immunoreactivities to 4 different nematodes were examined by ELISA. Seven clones pecifically recognized PWN while two clones recognized 4 nematodes. Our data suggested that PWN-AR1 is a PWN secretory enzyme while PWN is invading pine trees, Thus, PWN-AR1-Mabs could be used to develop diagnosis tools for PWN and its infected pine trees. (This work was supported by National Institute of Forest Science)

• A-Young Kim(Ilsong Institute of Life Science, Hallym University, Anyang, Korea, Department of Agricultural Biology, Seoul National University, Seoul, Korea)
• Angelina Fathia Osabutey(Ilsong Institute of Life Science, Hallym University, Anyang, Korea, Department of Biomedical Gerontology, Hallym University, Chuncheon, Korea)
• Kyungjae Andrew Yoon(Department of Agricultural Biology, Seoul National University, Seoul, Korea, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul Korea)
• Si-Hyeock Lee(Department of Agricultural Biology, Seoul National University, Seoul, Korea, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul Korea)
• Bo-Hye Choi(Ilsong Institute of Life Science, Hallym University, Anyang, Korea)
• Hyerim Han(Division of Forest Insect Pests & Disease, National Institute of Forest Science, Seou, Korea)
• Young Ho Koh(Ilsong Institute of Life Science, Hallym University, Anyang, Korea, Department of Agricultural Biology, Seoul National University, Seoul, Korea)