This work was performed to confirm the molecular discrimination through the nrITS1 sequences among 3 taxa of Scirpus L. sensu lato (s.l.) species. S. planiculmis represented only 2 base sequence variations with S. maritimus in spite that they showed different morphological features. The nucleotide sequences of the ITS1 region from S. planiculmis were shown to have 99.1% homology with S. maritimus and 60.4% homology with S. triqueter. Although the morphology of S. planiculmis is similar with S. triqueter, molecular basis of the size and sequences on ITS1 region were shown to have distinctive differences. For divergency investigation on same sites and metapopulation, sequencing was conducted on ITS1 region with partial 5.8S and 18S regions. All plants of each species collected at the same site had identical band size pattern and sequences. Intraspecific molecular divergency was not identified in spite that these species live in different wetland sites. The ITS1 sequences described here provided a powerful genetic tool for phylogenetic studies which was difficult by morphological identification as high rate of morphological plasticity

ABSTRACT
 I. INTRODUCTION
 II. Materials and Methods
 2.1 Plant materials
 2.2 Total genomic DNA extraction
 2.3 PCR and cloning
 2.4 Small scale (mini) preparation of plasmid DNA
 2.5 Sequence and phylogenetic analysis
 III. Results
 Ⅳ. Discussion
 ≫ Literature Cited

• Wol-Suk Jang(Department of Biology and Biomedical Science, Dong-A University)
• Hye-Sook Kang(Department of Biology and Biomedical Science, Dong-A University)
• In-Seop Han(School of Biological Science and Technology, College of Natural Science, Ulsan University)
• Sun-Hee Lee(Department of Biology and Biomedical Science, Dong-A University) Corresponding author