Origin of Red imported fire ant (RIFA : Solenopsis invicta) is Central America, a tropical climate region. It has settled in the invasive area, causing various problems. In recent, Solenopsis invicta were discovered in Busan in 2017 and then in 2018 at a construction site in Busan, Pyongtaek, Incheon and in Daegu. This study aims to confirm the origin of invasive colonies of Solenopsis invicta. We tried to test previously developed microsatellite markers so that we establish the tracing protocol for molecular epidemiology of RIFA. We justified 66 microsatellite markers already developed using DNA from the RIFA found in Incheon Harbor. As results, 30 markers were selected to facilitate amplification and fragment analysis.

• Sohee Kim(Department of Biology, Kunsan National University)
• Jongho Lee(Animal & Plant Quarantine Agency)
• Heungsik Lee(Animal & Plant Quarantine Agency)
• Seoungjin Lee(Animal & Plant Quarantine Agency)
• Youngjin park(Animal & Plant Quarantine Agency)
• Hyojoong Kim(Department of Biology, Kunsan National University)