Allomyrina dichotoma (order Coleoptera, family Scarabaeidae) is used for development of pharmaceuticals, pet or educational purposes and animal feedstuffs. The disease occurrence and distribution of Oryctes rhinoceros Nudivirus were investigated in Allomyrina dichotoma in Korea using PCR and analyzed the DNA seqeunces using BLAST(Basic Local Alignment Search Tool). The virus infected larvae were collected from 10 insect rearing farms in five different regions (Gyounggi, Chungbuk, Chungnam, Jeonnam, Daejeon). Frequency of OrNV virus infection appeared differently depending on the regions or rearing facilities (open field, vinyl house, indoor breeding system and etc.). The collected samples of Allomyrina dichotoma raised on open fields showed the highest possibilities of OrNV virus infection. The OrNV average infection rate of open fields rearing systems was 50.0%.

• Kyu-Won Kwak(Industrial Insect Division, National Institute of Agricultural Sciences, RDA, Korea, Department of Bio-fibers and materials Science, Kyungpook National University, Korea)
• Heui-Sam Lee(Industrial Insect Division, National Institute of Agricultural Sciences, RDA, Korea)
• Myung-Sae Han(Department of Bio-fibers and materials Science, Kyungpook National University, Korea)
• Sung-Hee Nam(Industrial Insect Division, National Institute of Agricultural Sciences, RDA, Korea)
• Kwanho Park(Industrial Insect Division, National Institute of Agricultural Sciences, RDA, Korea)