This study was performed to investigate the effect of types of oil (OVOIL vs. OIL) on B6D2F1 mice oogenesis. In this study, B6D2F1 F1 mice were used in order to maximize oogenesis. The expansion rate of cumulus cells (82.0%±0.2 vs. 78.0%±0.1), in vitro fertilization rate (92.0%±0.1 vs. 88.0%±0.1), developmental rate (91.0%±0.1 vs. 87.0%±0.2), blastocysts formation rate (56.0%±0.1 vs. 57.0%±0.1), and zona hatched rate(41.4%±0.2 vs. 24.0%±0.1) were not different between groups (NS; P>0.05). However, there was a significant difference in maturation rate; the OVOIL group showed higher maturation rate compared to that of the OIL group (96.0%±0.1 vs. 87.0%±0.1; P<0.05). In the blastocysts cell numbers, the total cell numbers (83.9±26.1 vs. 56.9±23.9), ICM cell numbers (15.7±8.8 vs. 6.3±3.5), TE cell numbers (68.3±25.7 vs. 50.7±24.1), % ICM (21.6%±0.1 vs. 12.7%±0.1), and the ratio of ICM:TE (1:6.2±6.5 vs. 1:10.3±7.0) were significantly higher in the OVOIL group than the OIL group (P<0.05).
These results suggested that it is expected to achieve the more developmental ability of B6D2F1 mice depending on the type of oil (OVOIL vs. OIL). In addition, the results can provide essential information for culture condition on B6D2F1 mice. Henceforth, thus, it is expected that these results herein might be used for in vitro culture of human embryos.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Animals
  B6D2F1 mice culture media and oil
  Preparation of mice oocytes and In Vitro Maturation (IVM)
  In Vitro Fertilization (IVF)
  In Vitro Culture (IVC) and differential fluorescence stainingof blastocysts
  Statistical analysis
 RESULTS
 DISCUSSION
 REFERENCES

• Dan-Bi Lee(Department of Animal Resources, Daegu University)
• Kee Sang Park(Department of Obstetrics and Gynecology, Kyungpook National University Hospital)
• Byoung Boo Seo(Department of Animal Resources, Daegu University) Correspondence