In this study, two epididymal spermatozoa recovery methods in relation to sperm number, motility, viability and acrosome reaction were examined. Seven bulls were castrated and 7 testicles with epididymides were transferred to the laboratoy. Epididymis in each bull was randomly used for flushing and mincing methods with semen extender (Optixcell, IMV, France). The recovered spermatozoa with adjusted sperm concentration to 40 × 106 cells/mL was diluted with optixcell and cryopreserved. In experiment 1, the difference in the total number of spermatozoa using flushing and mincing methods was insignificant (2570.0 and 2505.2 × 106 cells/mL, respectively). For experiment 2, the percentage of motile spermatozoa and motility parameters between flushing and mincing methods were studied through the use of sperm class analyzer after frozen-thawing. The percentage of total motile sperm between flushing and mincing methods was almost the same with 89.5±12.8 and 91.4±7.9%, respectively. The same is the case with experiment 3 wherein the viability and acrosomal integrity of frozen-thawed epididymal spermatozoa by flushing and mincing was insignificantly different. The results from the study showed that both flushing and mincing methods can be used for epididymal spermatozoa recovery in bull.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Ethics of animal experimentation
  Castration and recovery of epididymis
  Spermatozoa recovery by flushing method
  Spermatozoa recovery by mincing method
  Semen freezing
  Measurement of sperm motility
  Staining of frozen-thawed epididymal spermatozoa andevaluation of sperm viability and acrosomal membraneintegrity
 STATISTICAL ANALYSIS
 RESULTS AND DISCUSSION
 REFERENCES

• Sung-Sik Kang(Hanwoo Research Institute, NIAS, RDA)
• Ui-Hyung Kim(Hanwoo Research Institute, NIAS, RDA)
• Min-Hyeong Jeon(Hanwoo Research Institute, NIAS, RDA)
• Myung-Suk Lee(Hanwoo Research Institute, NIAS, RDA)
• Sang-Rae Cho(Hanwoo Research Institute, NIAS, RDA) Correspondence