The purpose of this study is to evaluate the fixed wing type domestic UAV for monitoring of algae bloom in aquatic environment. The UAV used in this study is operated automatically in-flight using an automatic navigation device, and flies along a path targeting preconfigured GPS coordinates of desired measurement sites input by a flight path controller. The sensors used in this study were Sequoia multi-spectral cameras. The photographed images were processed using orthomosaics, georeferenced digital surface models, and 3D mapping software such as Pix4D. In this study, NDVI(Normalized distribution vegetation index) was used for estimating the concentration of chlorophyll-a in river. Based on the NDVI analysis, the distribution areas of chlorophyll-a could be analyzed. The UAV image was compared with a airborne image at a similar time and place. UAV images were found to be effective for monitoring of chlorophyll-a in river.