This study was performed to develop a new natural antimicrobial materials by analyzing the effect of extracts obtained from Ten Lauraceae Species on the inhibitory activity against Propionibacterium acnes. The plant materials were collected from Wando and Jeju islands, and the antimicrobial activity of the crude extracts was examined by the agar diffusion method with different part (i.e., leaf and branch), solvents (i.e., distilled water, 80% ethanol, and 100% methanol) and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The control agents used were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 ㎎/disc. Altogether, extracts of 10 species used in the study showed inhibitory activity, which confirmed their antimicrobial action against acnes. Among these, leaves of Laurus nobilis L. which was extracted in 80% ethanol for 45 min showed the largest clear zone (19.8 ㎜). Leaves of L. nobilis L., showing highest antimicrobial activities among 10 species, were successively reextracted with n-hexane, chloroform, ethylacetate and n-butanol. As a results, in all fractions except butanol, clear zone above 10 ㎜ were formed. The ethyl acetate fraction showed the highest inhibitory activity (13.3 ㎜) and the inhibitory activity was significantly higher than that of crude extract (10.2 ㎜) and phenoxyethanol as a control (12.5 ㎜).

Abstract
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• 조주성((주)농우바이오) | Ju Sung Cho (Quality Control & Seed Tech Team, Nongwoo Bio CO., LTD.)
• 지래원(충북대학교 원예과학과) | Lai Won Chi (Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
• 장보국(충북대학교 원예과학과) | Bo Kook Jang (Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
• 정헌상(충북대학교 식품생명공학과) | Heon Sang Jeong (Department of Food Science and Biotechnology, Chungbuk National University)
• 이철희(충북대학교 원예과학과) | Cheol Hee Lee (Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) 교신저자