In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichum Nakai. The extracts were prepared using 0, 50, 70, and 100% prethanol A at 121℃, 1.2 atm for 15 minutes. To confirm effective extraction, the acteoside content of each extract was analyzed with the HPLC-PDA method. The antioxidant activity was evaluated using DPPH and ABTS scavenging activity assays, and the whitening activity was evaluated based on inhibitory activities on the protein and mRNA expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16 F10 cells. Each extract showed strong antioxidant and whitening activity. IC50 values of antioxidant activity from each extract were in order of 100%, 70%, 50%, and 0%. In addition, whitening activity inhibited the protein and mRNA expression of melanin synthesis factor, following the same pattern as antioxidant activity. In conclusion, water and prethanol A extracts of A. distichum showed effective antioxidant and whitening activity and are thus considered to be valuable materials for whitening cosmetics. The results of this study will also provide basic data for the safe and efficient production of A. distichum as a cosmetic material.

Abstract
  Introduction
  Materials and Methods
  Results and Discussion
  References

• Tae-Won Jang(Department of Medicinal Plant Resources, Andong National University)
• Ji-Soo Choi(Department of Medicinal Plant Science, Jungwon University)
• Hoi-Ki Kim(Fanipinkorea Co., Ltd)
• Eun-Ja Lee(Fanipinkorea Co., Ltd)
• Man-Wook Han(Fanipinkorea Co., Ltd)
• Ki-Beom Lee(Incheon Business Information Technopark. Biotechnology & Business Center)
• Do-Wan Kim(Department of Food Science and Industry, Jungwon University)
• Jae-Ho Park(Department of Pharmaceutical Science, Jungwon University) Corresponding author