Chrysanthemum boreale Makino is widely distributed in Korea, China, Japan and Southeast Asian countries. C. boreale is one of the herbs used for treating various inflammatory diseases in oriental medicine. The present study was conducted to identify biologically active compounds from the leaves and stems of C. boreale. We isolated two sesquiterpene sactones from the leaves and stems of C. boreale using silica gel column chromatography and recyclic high perfomance liquid chromatography. The lactones were characterized by their spectroscopic data (NMR, IR, MASS). These compounds were subjected to Farnesyl Protein Transferase (FPTase) inhibition, Nitric Oxide (NO) release inhibition and apoptosis inhibition. The structur of the following isolated compound were elucidated 8,10-o-Acetyl-2-methoxy-10-hydroxy-3,11(13)-guaiadiene-12,6-olide and 4,10-dihydroxy -8-o-Acetyl-2,11(13)-guaiadiene-12,6-olide. In the NO release inhibition assay, compound 2 showed strong activities, with an IC50 value of 7 μg/mL, whereas compound 1 did not exhibit significant activity with an IC50 value of over 14 μg/mL against murine macrophage.

Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 실험 재료 및 시약
  2.2. 재료 및 방법
  2.3. Apoptosis 저해실험
  2.4. 재료 및 시료의 추출
  2.5. 생리활성물질의 분리
 3. 결과 및 고찰
  3.1. 화합물 1과 2의 구조 규명
  3.2. 화합물 1과 2의 생리활성
 4. 결 론
 REFERENCES

• 이종록(대구한의대학교 제약공학과) | Jong Rok Lee (Department of Pharmaceutical Engineering, Daegu Haany University)
• 박문기(대구한의대학교 제약공학과) | Moon Ki Park (Department of Pharmaceutical Engineering, Daegu Haany University) Corresponding author