마치현 추출물에 대한 생리활성 소재로서 응용 가능성을 확인하고자 하였다. 마치현 추출물의 측정 결과, 플라보노이드, 폴리페놀 함량과 DPPH radical 소거능을 확인하였으며, 세포실험 결과 HaCaT, RAW 264.7, RBL-2H3 세포에서 유의한 세포독성은 나타나지 않았으며. H2O2에 의한 유발되는 산화적 스트레스에 대한 HaCaT 세포는 마치현 에탄올 100 μg/mL 농도에서 83% 보호효과가 확인되었다. RAW 264.7 세포에 대한 마치현 추출물의 항염 효과를 확인 결과 저농도에서도 nitric oxide 생성이 억제되었으며, S. aureus, S. epidermidis, P. acnes 균에서도 마치현 추출물의 농도 의존적으로 항균 활성이 확인되었다. 본 연구는 마치현 추출물의 항산화, 항염증 및 항균 효과를 가지는 생리 활성 물질로서 활용 가능성을 확인하였다.

The purpose of that was to investigate the potential of P. Herba extracts as phytonutrient active ingredients. In order to elucidate the P.Herba ethanol extracts were examined DPPH radical scavenging activity, NO production, protective effects against oxidative stress in HaCaT cells, anti-inflammatory activity, antimicrobial activity, anti-allergic effects, and inhibition of β-hexosaminidase expression. The antioxidative activity of the P. Herba extracts was compared, and the antioxidative activity of the ethanol extract was found to be superior. No significant cytotoxicity was observed in HaCaT, RAW 264.7, and RBL-2H3 cells. The protective effect of the extracts against oxidative stress induced by hydrogen peroxide (H2O2) was examined in HaCaT cells, and it was found to be 83% This concentration refers to which extract ethanol at 100 μ g/mL. The anti-inflammatory activity of the extracts was examined in RAW 264.7 cells, and NO production was suppressed even at low concentrations. In addition, the concentration-dependent antimicrobial activities of the extracts were demonstrated in several bacterial strains, such as those of S.aureus, S.epidermidis and P. acnes. Based on the findings from this study, Portulacae Herba extracts could be used as physiological active substance that possess antioxidative, anti-inflammatory, and antimicrobial properties.

요 약
 Abstract
 1. 서 론
 2. 실 험
  2.1. 시약 및 배양
  2.2. 항산화 활성 측정 방법
  2.3. 세포 생존율
  2.4. H2O2에 의하여 유발되는 산화적 스트레스 HaCaT 세포
  2.5. 항 알레르기 활성 측정
  2.6. Nitrite 생성 저해능 측정
  2.7. 균주 배양
 3. 결과 및 고찰
  3.1. 항산화 측정결과
  3.2. 마치현 추출물의 HaCaT 세포에 미치는 영향
  3.3. 마치현 추출물의 항 알레르기 활성
  3.4. 마치현 추출물의 항염 효과
  3.5. 항균력 분석
 4. 결 론
 References

• 곽정심(남부대학교 향장 미용학과) | Jeong Sim Gwak (Department of Cosmetology Science, Nambu University)
• 김춘득(남부대학교 향장 미용학과) | Chun-Dug Kim (Department of Cosmetology Science, Nambu University) Corresponding author