The present study was conducted to determine the effect of hexaconazole (HEX), a triazole fungicide, on the growth, yield, photosynthetic response and antioxidant potential in cucumber (Cucumis sativus L.) plants subjected to UV-B stress. UV-B radiation and HEX were applied separately or in combination to cucumber seedlings. The growth parameters were significantly reduced under UV-B treatment, however, this growth inhibition was less in HEX treated plants. HEX caused noticeable changes in plant morphology such as reduced shoot length and leaf area, and increased leaf thickness. HEX was quite persistent in inhibiting shoot growth by causing a reduction in shoot fresh and dry weight. HEX noticeably recovered the UV-B induced inhibition of biomass production. Significant accumutation in anthocyanin and flavonoid pigments in the leaves occurred as a result of HEX or UV-B treatments. HEX permitted the survival of more green leaf tissue preventing chlorophyll content reduction and higher quantum yield for photosystemⅡ under UV-B exposure. HEX treatment induced a transient rise in ABA levels in the leaves, and combined application of HEX and UV-B showed a significant enhancement of ABA content which activates H2O2 generation. UV-B exposure induced accumulation of H2O2 in the leaves, while HEX prevented UV-B induced increase in H2O2, indicating that HEX serves as an antioxidant agent able to scavenge H2O to protect cells from oxidative damage. An increase in the ascorbic acid was observed in the HEX treated cucumber leaves affecting many enzyme activities by removing H2O2 during photosynthetic processes. The activities of antioxidant enzymes including catalase(CAT), ascorbate peroxidase(APX), superoxide dismutase(SOD) and peroxidase(POD) in the leaves in the presence of HEX under UV-B stress were higher than those under UV-B stress alone. These findings suggest that HEX may participate in the enhanced tolerance to oxidative stress. From these results it can be concluded that HEX moderately ameliolate the effect of UV-B stress in cucumber by improving the components of antioxidant defense system.

Abstract
 1. Introduction
 2. Materials and Methods
  2.1. Plant material and growth conditions
  2.2. Ultraviolet-B treatments
  2.3. Growth measurements
  2.4. Determination of photosynthetic pigments
  2.5. Determination of flavonoid and anthocyanin contents
  2.6. Determination of abscisic acid content
  2.7. Chlorophyll fluorescence measurement
  2.8. Determination of H2O2 content
  2.9. Determination of antioxidant enzyme activity
  2.10. Determination of ascorbic acid
  2.11. Statistical analysis
 3. Results and Discussion
  3.1. Effect of HEX on morphological parameters under UV-B stress
  3.2. Effect of HEX on photosynthetic responses under UV-B stress
  3.3. Effect of HEX on antioxidant system under UV-B stress
 4. Conclusions
 References

• Tae-Yun Kim(Department of Biological Sciences, Pusan National University)
• Jung-Hee Hong(Department of Biological Sciences, Pusan National University) Corresponding author