An analytical method was developed for the determination of sedaxane in agricultural products using liquid chromatograph-tandem mass spectrometry (LC-MS/MS). The samples were extracted with acetonitrile and partitioned with dichloromethane to remove the interference, and then purified by using silica SPE cartridges to clean up. The analytes were quantified and confirmed by using LC-MS/MS in positive ion mode using multiple reaction monitoring (MRM). The matrix-matched calibration curves were linear over the calibration ranges (0.001- 0.25 μg/mL) into a blank extract with r 2>0.99. For validation, recovery tests were carried out at three different concentration levels (LOQ, 10LOQ, and 50LOQ, n=5) with five replicates performed at each level. The recoveries were ranged between 74.5 to 100.8% with relative standard deviations (RSDs) of less than 12.1% for all analytes. All values were consistent with the criteria ranges requested in the Codex guidelines (CAC/GL 40, 2003) and Food Safety Evaluation Department guidelines (2016). The proposed analytical method was accurate, effective and sensitive for sedaxane determination in agricultural commodities.

ABSTRACT
 Materials and Methods
  시약 및 재료
  표준원액 및 표준용액의 조제
  추출 및 액액분배
  카트리지 정제
  LC-MS/MS 분석조건
  시험법의 검증
 Results and Discussion
  기기선정 및 분석조건 확립
  추출 및 분배조건 확립
  카트리지 정제법 확립
  시험법 검증
  실험실간 검증
 국문요약
 Reference

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• 도정아(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Jung-Ah Do (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) Correspondence to
• 박신민(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Shin-Min Park (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 이한솔(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Han Sol Lee (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 박지수(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Ji-Su Park (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 신혜선(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Hye-Sun Shin (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 장동은(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Dong Eun Jang (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 최영내(한국식품과학연구원) | Young-Nae Choi (Korea Advanced Food Research Institute)
• 정용현(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Yong-hyun Jung (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 이강봉(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Kangbong Lee (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)