Validamycin A is an aminoglycoside fungicide produced by Streptomyces hygroscopicus that inhibits trehalase. The purpose of this study was to develop a method for detecting validamycin A in agricultural samples to establish MRL values for use in Korea. The validamycin A residues in samples were extracted using methanol/ water (50/50, v/v) and purified with a hydrophilic-lipophilic balance (HLB) cartridges. The analyte was quantified and confirmed by liquid chromatograph-tandem mass spectrometer (LC-MS/MS) in positive ion mode using multiple reaction monitoring (MRM). Matrix-matched calibration curves were linear over the calibration ranges (0.005~0.5 ng) into a blank extract with R2 > 0.99. The limits of detection and quantification were 0.005 and 0.01 mg/kg, respectively. For validation validamycin A, recovery studies were carried out three different concentration levels (LOQ, LOQ × 10, LOQ × 50, n = 5) with five replicates at each level. The average recovery range was from 72.5~118.3%, with relative standard deviation (RSD) less than 10.3%. All values were consistent with the criteria ranges requested in the Codex guidelines (CAC/GL 40-1993, 2003) and the NIFDS (National Institute of Food and Drug Safety) guideline (2016). Therefore, the proposed analytical method is accurate, effective and sensitive for validamycin A determination in agricultural commodities.

ABSTRACT
 Materials and Methods
 Results and Discussion
 국문요약

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• 도정아(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Jung-Ah Do (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) Correspondence to
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• 박신민(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Shin-min Park (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 조성민(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Sung Min Cho (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 신혜선(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Hye-Sun Shin (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 장동은(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Dong Eun Jang (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 조명식(광주지방식품의약품안전청 유해물질분석과) | Myong-Shik Cho
• 정용현(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Yong-hyun Jung (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
• 이강봉(식품의약품안전처 식품의약품안전평가원 식품위해평가부 잔류물질과) | Kangbong Lee (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)