Microorganisms producing bioemulsifier were isolated from the sea water in Pusan coastal area. The isolated strain which had the highest emulsification activity and stability was identified as the genus Acinetobacter from the results of morphological, cultural and biochemical tests and named Acinetobacter sp. EL-C6 for convenience. The compositions of optimum medium for emulsification of crude oil by Actnetobacter sp. EL-C6 were crude oil 2.0%, NH_4NO_3 0.2%, K_2HPO_4 0.01%, MgSO_4·7H_2O 1.0%, CaCl_2·2H_2O 0.1% and NaCl 3.0% at initial pH 7.5 and 30℃, respectively. The cultivation for emulsification of crude oil was carried out in 500㎖ shaking flask containing 100㎖ of the optimum medium at 30℃. The highest emulsification was observed after 5 days. The utilization on the various hydrocarbon of the Acinetobacter sp. EL-C6 showed that utilization of n-alkane compounds were better than that of aromatic compounds. Among the petroleum compounds, crude oil was best utilized by the Actnetobacter sp. EL-C6.

• 손홍주(부산대학교 환경문제연구소) | Hong-Joo Son (Institute of Environmental Studies, Pusan National University)
• 차미선(부산대학교 미생물학과) | Mi-Sun Cha (Department of Microbiology, Pusan National University)
• 김희구(부산대학교 미생물학과) | Hee-Gu Kim (Department of Microbiology, Pusan National University)
• 이건(부산대학교 미생물학과) | Geon Lee (Department of Microbiology, Pusan National University)
• 이상준(부산대학교 미생물학과) | Sang-Joon Lee (Department of Microbiology, Pusan National University)