The culture supernatant of Lactobacillus casei CJNU 0588 was prepared from a culture incubated in whey broth (10%, w/v). The culture supernatant stimulated the growth of Bifidobacterium longum subsp. infantis KCTC 3127 and Lactobacillus acidophilus KCTC 3145 which are recognized as human gut beneficial bacteria, whereas inhibited that of Clostridium difficile KCTC 5009 as a gut pathogenic bacterium and Escherichia coli JM109. In co-culture test, L. casei CJNU 0588 cells slightly stimulated the growth of B. longum subsp. infantis KCTC 3127 and L. acidophilus KCTC 3145 but mildly inhibited that of C. difficile KCTC 5009 and E. coli JM109. Therefore L. casei CJNU 0588 strain might positively function to gut microbiota and can be used for gut health-promoting probiotics.

Abstract
 Introduction
 Materials and Methods
  Preparation of L. casei CJNU 0588 culture supernatant
  Effect of L. casei CJNU 0588 culture supernatanton the growth of gut bacteria
  Effect of L. casei CJNU 0588 cells on the growth ofgut bacteria
  Statistical analysis
 Results and Discussion
 References

• Jo-Eun Kang(Department of Biotechnology, Korea National University of Transportation)
• Gi-Seong Moon(Department of Biotechnology, Korea National University of Transportation) Corresponding author