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Porphyromonas gingivalis lipopolysaccharide stimulates vascular smooth muscle cell migration through signal transducer and activator of transcription 3-mediated matrix metalloproteinase-9 expression KCI 등재후보
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- URLhttps://db.koreascholar.com/Article/Detail/367545
Periodontal diseases have been associated with the development of cardiovascular diseases. Accumulating evidences have indicated that Porphyromonas gingivalis , a major periodontopathic pathogen, plays a critical role in the pathogenesis of atherosclerosis. In the present study, we demonstrated that P. gingivalis lipopolysaccharide (LPS) increases the mRNA and protein expression of matrix metalloproteinase-9 (MMP-9) in rat vascular smooth muscle cells. We showed that the MMP-9 expression induced by P. gingivalis LPS is mediated by the activation of signal transducer and activator of transcription 3 (STAT3) in vascular smooth muscle cells. Furthermore, the inhibition of STAT3 activity reduced P. gingivalis LPS-induced migration of vascular smooth muscle cells. Overall, our findings indicate that P. gingivalis LPS stimulates the migration of vascular smooth muscle cells via STAT3-mediated MMP-9 expression.
Materials and Methods
1. 실험재료
2. 혈관평활근세포배양
3. RNA 분리와 역전사 중합효소연쇄반응(reverse transcription-polymerase chain reaction, RT-PCR)
4. 실시간 중합효소연쇄반응(real-time quantitative reverse transcription-PCR, real-time qRT-PCR )
5. Western immunoblot analysis
6. 혈관평활근세포의 이동성 분석(scratch wound migration assay)
7. 통계처리
Results
1. P. gingivalis LPS가 혈관평활근세포의 MMP-9 발현에 미치는 영향
2. P. gingivalis LPS가 유도한 MMP-9의 발현에 JAK2/STAT3 신호전달기전의 관련성
3. JAK2/STAT3 저해제가 P. gingivalis LPS가 유도하는 혈관평활근세포의 이동능에 미치는 영향
Discussion
References
