This study was conducted to elucidate microplastics detection of freshwater ecosystems in Geum river. Samples are collected at 6 points in freshwater, 5 points in fishes. Freshwater was sampled 100 L per each points and fish species were Opsariichthys uncirostris amurensis, Hemibarbus labeo, Pseudogobio esocinus, Zacco platypus, Micropterus salmoides and Cyprinus carpio. FTIR analyis was adopted to identify microplastic types. Extracted microplastics were PES (polyester), PE (polyethylene), PP (polypropylene), PET (polyethylene terephthalate), PVC (Polyvinyl chloride) in freshwater, and PES, PE, PP, PET, PVC in fishes. Our results were expected to be used basic research information for further study in microplastics of freshwater ecosystems.