Insecticide Resistant Management (IRM) and Positive List System (PLS) is the main keyword in current insect pest management. So we are trying to develop a integrated insect pests control prescription issuing system based on big data which linked National Crop Pest Management System (NCPMS). To manage the insecticide resistance, IRAC suggest some tips for effective control : choosing the effective insecticide and alteration of these insecticides based on MOA. Then, how to distinguish the effective or non-effective insecticide. Until now, bioassay is the right answer. However that is time-consuming, labor-intensive and costly process. So we have been steadily advancing molecular based insecticide resistance diagnosis for supporting and/or substitution of classical bioassay. And it will link to PLS. Here we discuss more detailed methodology of molecular based insecticide resistance diagnosis and its application for the insect pests control prescription issuing system.