Ambrosia artemisiifolia is native in North America and an invasive alien species in East Asia and Europe. This plant causes economic losses such as reducing agricultural production and producing severe allergenic pollen. Recently, there was an effort to control this alien plant chemically and mechanically in South Korea, but they are neither sustainable nor environmentally-friendly control strategies. Epiblema sugii Kawabe 1976 (Lepidoptera: Tortricidae) is known as a potential biological control agent of A. artemisiifolia. In order to control this species using a biological control method, we investigated overwintering structures and spatial distributions of E. sugii in A. artemisiifolia colony as an initial step.