Monochamus alternatus which is major vector for Bursaphelenchus xylophilus is main pest for pine trees. Moreover, their larvae mainly feed on the phloem of the host during the growth period, and this process makes host debilitate. The reason why the epidemiological investigation of that damage caused by Monochamus alternatus is difficult is that the adult and the larva remains very rarely in the host. Even if the larvae remain in the host, it is not easy to distinguish them from other cerambycidae coleopterans without expertise. For the above reason, we introduce the method of field epidemiological investigation for Monochamus alternatus using the remaining larva frass in the host with Recombinase Polymerase Amplification (RPA) method.

• Deok Jea Cha(Forest Biomaterials Research Center, National Institute of Forest Science)
• Dong Soo Kim(Forest Biomaterials Research Center, National Institute of Forest Science)