This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of 9,889 ± 3 μg/L to 12,909 ± 2 μg/L at 45 days and 29,256 ± 4 μg/L at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to 83,751 ± 2 μg/L after 45 days from its initial value of 161,787 ± 1 μg/L and to 79,055 ± 7 μg/L at 90 days.

Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 균주 배양
  2.2. 아미노산 분석
 3. 결과 및 고찰
 4. 결 론
 REFERENCES

• 오태석(공주대학교 식물자원학과) | Tae-Seok Oh (Department of plant Resources, Kongju National University)
• 박윤진(공주대학교 두과녹비자원연구센터) | Youn-Jin Park (Green Manure and Legumes Resource Center, Kongju National University)
• 김태권(공주대학교 두과녹비자원연구센터) | Tae-Kwon Kim (Green Manure and Legumes Resource Center, Kongju National University)
• 안승원(공주대학교 원예학과) | Seoung-Won Ann (Department of plant Resources, Kongju National University)
• 김창호(공주대학교 식물자원학과) | Chang-Ho Kim (Department of plant Resources, Kongju National University)
• 조용구(공주대학교 식물자원학과) | Yong-Koo Cho (Department of plant Resources, Kongju National University)
• 장명준(공주대학교 식물자원학과) | Myoung-Jun Jang (Department of plant Resources, Kongju National University) Corresponding author