The bacteriophage from the fresh oyster, Crassostrea Vrginiea which is specific to the marine bacterium was isolated and characterized. Among the four different vibrio species and the five different serotypes of. Vibrio parahaemolyticus host strains tested, only two strains of Vibrio parahaemolyticus possessing K17, K52 antigens were highly sensitive to the phage. The size of the isolated plaque was 0.4㎜ and the electron microscopic head size of the isolated phage was about 67 nm long and 83 nm wide. PFU/㎖ was 1.25×10^11. The phage was sensitive to chloroform but resistant to acetone or methanol. The assay of the iseoted phage nucleic acid was deoxyribonucleic acid. The restriction enzyme pattern showed 14 fragments from Hind Ⅲ and 4 fragments from Eco R I. Two different antigenic groups showed similar restriction enzyme patterns.

• Young Hee KIM(Dept. of Microbiology, Dongeui University) | 김영희