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Tannic acid-induced apoptosis in FaDu hypopharyngeal squamous cell carcinoma KCI 등재후보
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/373965
Tannic acid (TA) is a water-soluble polyphenol compound found in various herbal plants. We investigated the chemopreventive effects of TA on FaDu hypopharyngeal squamous carcinoma cells. In an 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay, TA showed dose-dependent cytotoxicity with a half maximal inhibitory concentration (IC50) of 50 μM. Cell cycle analysis and immunofluorescence imaging demonstrated that under lowdose (25 μM) treatment, FaDu cells were arrested in G2/M phase, and as the dose of TA was increased, apoptosis was induced with the increase of cell population at sub-G1 phase. The expressions of various cyclins, including cyclin D1 and cyclin-dependent kinases (CDK-1 and CDK-2), were down-regulated at low doses of TA, whereas apoptotic effectors such as cleaved caspase 3, cleaved caspase 7, and poly (ADP-ribose) polymerase (PARP) were expressed in a dose-dependent manner in Western blotting. In addition, TA-induced apoptosis of FaDu cells might be mediated by the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase pathway, with the upregulation of p-AKT/p-PKB (phosphorylated protein kinase B) and p-ERK. Overall, our data support the hypothesis that TA is a potential candidate agent for the treatment of hypopharyngeal cancer.
Materials and Methods
1. Materials and reagents
2. Cell culture
3. MTT assay
4. Cell cycle analysis
5. Western blotting
6. Immunofluorescence imaging
Results
1. The cytotoxicity of tannic acid on FaDu cell
2. Tannic acid induced cell cycle arrest and apoptosis
3. Tannic acid suppressed cell cycle regulatoryproteins
4. Tannic acid-induced apoptosis was throughcaspase-dependent pathway
Discussion
References
