논문 상세보기

Anti-atopic dermatitis effect of the soybean fermented by B. amyloliquefaciens via inhibiting IL-31 KCI 등재

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/374312
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
충북대학교 동물의학연구소 (Research Institute of Veterinary Medicine, Chungbuk National University)
초록

The occurrence of allergic disease has increased harmfully in the last few decades. Atopic dermatitis (AD) is an allergic inflammation disorder characterized by itchy, red, swollen, cracked skin. Although the pathogenesis of AD is not fully understood, it is assumed that deregulation of T-helper 1 (Th1) and T-helper 2 (Th2) immune responses, a predominance of allergen-specific IgE, and interrupted epidermal barrier function are keys to the pathogenic mechanism. Activated T helper 2 (Th2) immune function is hallmark of various allergic diseases. Oxidative stress implicated in cutaneous damage in various inflammatory skin diseases.
We investigated the effect of fermented soybean (SCGB1) on the improvement of AD. Soybean fermentation was carried out using B. amyloliquefaciense SCGB1 (KCCM11964P), which is known to produce of natural antibiotics. And then, we experiment of SCGB1 and soybean powder (NC) in DNCB-induced AD model. Mice were respectively oral administration of variety dose for 14 days. As a results, it was confirmed that serum Immunoglobulin E (IgE) expression was dose-dependently decreased in SCGB1 and NC compared to negative control, and it was reduced the skin pruritus inducing factor that Interleukin-31 (IL-31) mRNA level. In addition, the inflammatory cells were infiltration in skin for histological analysis. As a result, it reduced that epidermal hyperplasia, cancellation and aveolarization compared to negative control. These results suggest that SCGB1 may be effective for prevention and treatment of AD.

목차
Introduction
 Materials and Methods
  사용균주
  청국장 추출물 제조
  실험 동물
  검체 채취
  SCORAD test
  혈액을 통한 cytokine 분석
  조직에서의 real-time PCR
  조직학적 검사
  통계처리
 Results
  체중 변화량 측정
  피부형태 변화 분석
  SCORAD test
  혈액을 통한 cytokine 분석
  조직에서의 real-time PCR
  조직학적 검사
 Discussion
 References
저자
  • Sunhwa Hong(Center for Animal Resources Development, Wonkwang University)
  • Sung Woo Hwang(Center for Animal Resources Development, Wonkwang University)
  • Hee-Jong Yang(Microbial Institute for Fermentation Industry (MIFI))
  • Do-Youn Jeong(Microbial Institute for Fermentation Industry (MIFI))
  • Okjin Kim(Center for Animal Resources Development, Wonkwang University) Corresponding author