Acute myocardial infarction (AMI) is considered the major cause of mortality in the world. Tremendous animal studies are performed to develop novel therapeutics, and this study aimed to induce porcine myocardial infarction model by using polyethylene terephthalate (PET). Coronary guidewire was placed in left anterior descending artery (LAD). The balloon angioplasty catheter was inserted at the back of the PET. The balloon catheter was carefully pushed forward, until the balloon marker was located in mid-LAD. Coronary angiography was performed pre- and post-occlusion at 28 days by C-arm. Histologic analysis of heart tissue was performed 28 days after inducing AMI. Thirty three pigs were anesthetized and underwent percutaneous coronary catheterization. All pigs were successfully embolized in mid-LAD by PET. Fifteen pigs died due to ventricular fibrillation during post-anesthetic recovery time, and overall experiment mortality was 45.5%. In 2,3,5- triphenyl tetrazolium chloride staining, gross finding of the ischemic heart lesion showed firm and white area of infarction associated with the apex and left ventricular posterior wall. Infarct on H&E-stained sections demonstrated a region without myocytes and rich with cardiomyocyte with atypical nuclei. Successful induction of AMI by using PET may provide the pathophysiological information of ischemic heart disease and improvement of therapy development for AMI.

Introduction
 Materials and Methods
  Animal preparation
  Materials
  Preparation of polyethylene terephthalate occlude
  Anesthesia
  Protocol for AMI induction
  Electrocardiographic monitoring for AMI and arrhythmiaconfirmation
  Evaluation of left ventricular systolic function by usingtwo-dimensional transthoracic echocardiography
  Pathological analysis of infarcted lesion
  Statistical analysis
 Results
  AMI induction using PET
  Coronary angiographic findings
  Echocardiographic findings 1 hour and 4 weeks afterAMI compared with baseline
  Gross and histological findings
 Discussion
 Conclusion
 References

• Yong Sook Kim(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital)
• Nan Yeol Kim(Departments of Thoracic and Cardiovascular Surgery and Cardiology, Wonkwang University School of Medicine)
• In-Ho Bae(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital/Korea Cardiovascular Stent Research Institute)
• Jun Kyu Park(CGBio Co. Ltd)
• Dae Sung Park(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital/Korea Cardiovascular Stent Research Institute/Research Institute of Medical Sciences, Chonnam National University)
• Jae Won Shim(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital/Korea Cardiovascular Stent Research Institute)
• Wan Suk Kang(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital)
• Han Byul Kim(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital)
• Bong-Seok Song(Futuristic Animal Resource and Research Center & National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology)
• Bon-Sang Koo(Futuristic Animal Resource and Research Center & National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology)
• Kang-Jin Jeong(Futuristic Animal Resource and Research Center & National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology)
• Sun-Uk Kim(Futuristic Animal Resource and Research Center & National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology)
• Hyun Kuk Kim(Department of Internal Medicine and Cardiovascular Center, Chosun University Hospital, University of Chosun College of Medicine)
• Sung Soo Kim(Department of Internal Medicine and Cardiovascular Center, Chosun University Hospital, University of Chosun College of Medicine)
• Min Chul Kim(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital)
• Doo Sun Sim(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital)
• Young Joon Hong(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital)
• Youngkeun Ahn(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital)
• Kyung Seob Lim(Futuristic Animal Resource and Research Center & National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology) Corresponding author
• Myung Ho Jeong(Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare/Cardiovascular Research Center, Chonnam National University Hospital/Korea Cardiovascular Stent Research Institute) Corresponding author