Soil acidity or alkalinity are serious limitations for crop production. The purpose of this study was to clarify the negative effects of extreme pH stress (low and high) on alfalfa vegetative growth (VG) and biomass accumulation (BA). Two-week-old alfalfa seedlings were exposed to different pH (4.0, 4.5, 7.0, 8.0 and 8.5, respectively) levels for 72 hours. Alfalfa grown at pH 4.0 and 8.5 significantly reduced VG and BA, wherein as neutral pH (7.0) comparably exhibited better growth and biomass yield. These results indicate that extreme acidic or alkaline level are critical limiting factors for growth and biomass yield in alfalfa.

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Plant growth and treatments
    2. Determination of alfalfa growth and biomass accumulation
    3. Measurement of chlorophyll content
    4. Statistical analysis
Ⅲ. RESULTS AND DISCUSSION
Ⅳ. Conclusion
Ⅴ. ACKNOWLEDGMENTS
Ⅵ. REFERENCES

• Ki-Won Lee(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
• Sang-Hoon Lee(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
• Yowook Song(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
• Hee Jung Ji(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
• Ki-Yong Kim(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
• Gi Jun Choi(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
• Eun A Lim(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
• Md. Atikur Rahman(Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Corresponding author