The purpose of this study was to investigate the antimicrobial activity of the ethanol extract of Garcinia mangostana L. (mangosteen) against Cutibacterium acnes (6 strains) and Staphylococcus aureus (6 strains). The antimicrobial activity of the mangosteen extract was evaluated based on its minimal bactericidal concentration. Cytotoxicity of the mangosteen extract against human embryonic kidney 293 (HEK 293) cells was determined using the cell counting method. The data showed that the mangosteen extract was not toxic to HEK 293 cells at a concentration of up to 16 μg/mL and killed 87.0% and 99.9% of C. acnes and S. aureus after 10 minutes and 1 hour of treatment, respectively. These results suggest that ethanol extract of mangosteen can be used as an anti-acne agent.

Introduction
Materials and Methods
    1. 세포독성실험
    2. 세균 및 세균 배양
    3. 최소살균농도(minimum bactericidal concentration, MBC) 값 측정
Results
    1. HEK 293 세포주에 대한 세포 독성
    2. 망고스틴 추출물의 항균 활성 효과
Discussion
References

• Yun Kyong Lim(Korean Collection for Oral Microbiology, College of Dentistry, Chosun University, Department of Oral Biochemistry, College of Dentistry, Chosun University)
• So Young Yoo(Medi Bio Lab Co., Ltd.)
• Soon-Nang Park(Korean Collection for Oral Microbiology, College of Dentistry, Chosun University, Department of Oral Biochemistry, College of Dentistry, Chosun University)
• Dae Sung Lee(Medi Bio Lab Co., Ltd.) Correspondence to
• Joong-Ki Kook(Korean Collection for Oral Microbiology, College of Dentistry, Chosun University, Department of Oral Biochemistry, College of Dentistry, Chosun University) Correspondence to