PURPOSES : Korea is extremely vulnerable to the effects of climate change and earthquakes. When disasters occur, road infrastructure becomes a vital carriageway for moving people, emergency resources, and rescue teams. By designating disaster response routes in advance, the effect of the disaster can be reduced by ensuring a secure and quick moving space for emergency resources and relief activities. However, Korea has not established disaster response routes yet. Thus, this paper aims to prescribe guidelines to select appropriate disaster response routes by using Space Syntax while assuming a flooding disaster, as they occur most frequently and cause the biggest damage in terms of the recovery cost. METHODS : Based on the literature review, there are various approaches to selecting disaster response routes such as vulnerability, reliability, and redundancy based analyses. This study adopts the vulnerability-based approach, where the most vulnerable sections of roadway are selected for the disaster response route. RESULTS: We successfully developed guidelines for selecting a disaster response route assuming a flooding disaster. CONCLUSIONS: The analysis of the disaster response route indicated that the pre-determined disaster response route could minimize the damage due to the disaster, as it provides basic mobility and facilitates the swift movement of refugees and rescue teams.