Tissue engineering has been rapidly developed in oral and maxillofacial reconstruction. Biocompatible scaffold from chemically composites seeded with stem cells is essential and several growth factors for bone formation and angiogenesis are also required. To overcome limited activity of new bone formation with scaffolds, several biomechanical stimulation methods on cells have been made to grow cells in scaffold. Several bioreactors have been developed for real tissue growth in culture laboratory. In addition to biological stimulants like BMP, growth factors and exogenous drugs, biomechanical stimulation technique has also been known as an effective method in cell differentiation. We developed our own bioreactor with tensile mechanical strains. Then we tested with it for detection of suitable biomechanical effect on the cell differentiation and proliferation. And we also compared the results with the effect of low intensity pulsed ultrasound (LIPUS). Mechanical strain group showed more rapid reaction with cell differentiation and proliferation than non-mechanical strain group. Mechanical strain groups stimulated with 0.5∼0.7Hz for 6 hours and 8 hours showed more active cell differentiation than the group with 0.5∼0.7Hz for 2.5 hours tensile strain stimulation. Group of LIPUS also showed more rapid reaction in cell differentiation and proliferation. LIPUS with 3MHz showed more cell reaction than the LIPUS group with 1MHz. Our results showed the positive effect on differentiation and proliferation of cell with mechanical tensile strain, LIPUS both.

• 최용식(부산대학교 치의학전문대학원 구강악안면외과학교실) | Yong-Sik Choi (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
• 금동호(부산대학교 치의학전문대학원 구강악안면외과학교실) | Dong-Ho Geum (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
• 황대석(부산대학교 치의학전문대학원 구강악안면외과학교실) | Dae-Seok Hwang (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
• 유미현(부산대학교 치의학전문대학원 구강병리학교실, BK21 PLUS, 치의생명과학 연구소) | Mi-Heon Ryu (Dept. of Oral Pathology, BK21 PLUS project, Dental and Life Science Institute, School of Dentistry, Pusan National University)
• 김규천(부산대학교 치의학전문대학원 구강해부학교실) | Gyoo-Cheon Kim (Dept. of Oral Anatomy, School of Dentistry, Pusan National University)
• 김욱규(부산대학교 치의학전문대학원 구강악안면외과학교실) | Uk-Kyu Kim (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) Correspondence