Through time-series BV CCD photometry of the open cluster NGC 225 region, we have detected 30 variable stars including 22 new ones. They are five δ Scuti-type variable stars, a slowly pulsating B star, six eclipsing binary stars and 18 semi-long periodic or slow irregular variables, respectively. We have performed multiple-frequency analysis to determine pulsation frequencies of the δ Scuti-type stars and a slowly pulsating B star, using the discrete Fourier transform and linear least-square fitting methods. We also have derived the periods and amplitudes of 6 eclipsing binaries and a long-period variable star from the phase tting method, and presented the light curves of all variable stars. A slowly pulsating B star is a member of NGC 225, but δ Scuti-type stars are not members from the positions in the color-magnitude diagram and the radial distancies from the center of the cluster. From Dias et al. (2014, A&A, 564, 79), only three variable stars including the slowly pulsating B star are members of clusters: two are in NGC 225 and one is in Stock 24. But a variable star in Stock 24 is not a member of the cluster because of its position of color-magnitude diagarm.