This is the second paper on a systematic search for variable stars in Cyg OB3 region. In this paper we present the observational properties of 46 variable stars found in the region. Among them, 27 are eclipsing binaries and others are semi-long periodic or slow irregular variables. Only 5 are known variables and the rest are newly discovered ones. We have obtained the period and amplitude of 20 eclipsing binaries using the phase fitting method, and present the light curves of 46 variable stars.