논문 상세보기
A study on the inflammatory response induced by LPS of the Arthrospira platensis ethanol extract KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/385835
pp.966-974
Arthrospira platensis has been reported to contain a variety of substances such as phycocyanin, β-carotene, vitamin E and other carotenoids. In this study, zebrafish were treated with indoor cultivation spirulina ethanol extracts(ICAE) to determine toxicity(coagulation rate, hatching rate, heart rate). We used the DCFH-DA staining method to detect the effect of reactive oxygen species(ROS) generation on lipopolysaccharide(LPS)-induced zebrafish embryos ROS various concentrations(0.01, 0.05, 0.1, 0.5mg/ml) of ICAE. Cell toxicity was measured by WST-1 assay on RAW 264.7 macrophage cell line. Also, measured the inhibitory effect of nitric oxide(NO) and prostaglandin E₂(PGE₂) production in RAW264.7 macrophages induced by LPS at various concentrations of ICAE. The results of embryo coagulation rate, hatching rate, heart rate of zebrafish at various(0.01, 0.05, 0.1mg/ml) of ICAE was no toxicity. The ICAE treated group had an inhibitory effect on NO and PGE₂ production compared and decreased with concentration. The results of this study ethanol extract of Arthrospira platensis has an anti-inflammatory effect and suggest that is worthy of use cosmetics for skin protection.
1. INTRODUCTION
2. MATERIALS AND METHODS
2.1. Materials
2.2. Extraction method
2.3. Zebrafish maintenance
2.4. Toxicity measurement of ICAE inzebrafish embryo and larvae
2.5. Measurement of LPS-induced ROSproduction in zebrafish
2.6. Cell culture
2.7. Cell viability assay
2.8. Measurement of NO production inRAW 264.7 macrophages
2.9. Measurement of PGE2 production inRAW 264.7 macrophages
2.10. Statistical analysis
3. RESULTS AND DISCUSSION
3.1. Toxicity of ICAE in zebrafish
3.2. ROS production in LPS-inducedzebrafish
3.3 Cell viability
3.4. NO production in RAW 264.7macrophages
3.5. PGE₂reduction effect of ICAE
4. CONCLUSION
References
