Chlorella gloriosa (Chlorellaceae, Trebouxiophyceae) was isolated from seawater off the coast of the Dokdo Islands in Korea. An axenic culture was established using the streak-plate method on f/2 agar media supplemented with antibiotics, allowing identification of the isolate by morphological, molecular, and physiological analyses. The morphological characteristics observed by light and electron microscopy revealed typical morphologies of C. gloriosa species. The molecular phylogenetic inference drawn from the small-subunit 18S rRNA sequence verified that the microalgal strain belongs to C. gloriosa. Additionally, gas chromatography-mass spectrometry analysis showed that the isolate was rich in nutritionally important omega-3 and -6 polyunsaturated fatty acids and high-performance liquid chromatography analysis revealed that the high-value antioxidants lutein and violaxanthin were biosynthesized as accessory pigments by this microalga, with arabinose, galactose, and glucose as the major monosaccharides. Therefore, in this study, a Korean marine C. gloriosa species was discovered, characterized, and described, and subsequently added to the national culture collection.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    1. Sample collection and isolation
    2. Morphological identification
    3. Molecular identification
    4. Gas Chromatography/Mass Spectrometry(GC/MS) analysis
    5. High Performance Liquid Chromatography(HPLC) analysis
    6. Elemental analysis
    7. Carbohydrate analysis
RESULTS
DISCUSSION
REFERENCES

• Nam Seon Kang(Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea)
• Jung A Lee(Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea)
• Hyeong Seok Jang(Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea)
• Kyeong Mi Kim(Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea)
• Eun Song Kim(Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea)
• Moongeun Yoon(Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea)
• Ji Won Hong(Department of Taxonomy and Systematics, National Marine Biodiversity Institute of Korea) Corresponding author