We present JHKS near-infrared CCD photometric study for the Galactic open clusters NGC 1641 and NGC 2394. These clusters have never been studied before, and we provide, for the first time the cluster parameters; reddening, distance, metallicity and age. NGC 1641 is an old open cluster with age 1.6 ± 0.2 Gyr, metallicity [Fe/H]= 0.0 ± 0.2 dex, distance modulus(m-M)0=10.4 ± 0.3 mag(d=1.2 ± 0.2 kpc), and reddening E(B-V)=0.10±0.05 mag. The parameters for the other old open cluster NGC 2394 are estimated to be age=1.1±0.2 Gyr, [Fe/H]=0.0±0.2 dex, (m-M)0=9.1±0.4 mag(d=660±120 pc), and E(B-V)=0.05±0.10 mag. The metallicities and distance values for these two old open clusters are consistent with the relation between the metallicities and the Galactocentric distances of other old open clusters. We find the metallicity gradient of 53 old open clusters including NGC 1641 and NGC 2394 to be Δ[Fe/H]/ΔRgc=-0.067 ± 0.009 dex kpc-1.