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Differentiation potential of mesenchymal stem cells through electrical stimulation KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Electrical stimulation (ES) is known to guide the development and regeneration of many tissues. Use of low-frequency ES for therapeutic purposes has been increasing during the last decades. Mesenchymal stem cells (MSCs) represent an appealing alternative cell source for cartilage repair. There are studies that induce differentiation into cartilage cells by treating the growth factors in stem cells or altering the properties of stem cells by genetic modification. In this study, we exposed equine adipose tissue-derived MSCs (eAD-MSCs) to ES and assessed changes in the chondrogenic differentiation potential. The cells obtained from equine adipose tissue attached to culture plates and expanded in vitro. Flow cytometric analysis at third passage indicated that the cells were strongly positive for CD44, CD90, and CD105, but negative for CD13, CD34, and CD45. Next, ES was applied to eAD-MSCs cultured under condition of high-density micromass under ES of 10 V/cm, with duration of 10 ms and a frequency of 2.0 Hz for three days. Gene expression of chondrogenic markers such as collagen type II, Aggrecan, and Sox9 was analyzed at three days of ES. As a result, we observed the differentiation potential of eAD-MSCs into chondrocytes by specific ES in absence of exogenous growth factors. We also found that ES upregulated the expression of heat shock protein 70, which affects cartilage formation. This study may contribute to the differentiation of MSCs into chondrogenic lineage under specific ES condition.

목차
Abstract
서 론
재료 및 방법
    말 지방조직 유래 성체줄기세포 분리 및 배양
    세포 증식능 분석(cumulative population doubling levelanalysis, CPDL)
    정량적 중합효소연쇄반응(quantitative real-time RT-PCR,qRT-PCR)을 이용한 유전자 분석
    성체줄기세포의 유세포 분석
    성체줄기세포의 중배엽세포 분화 유도 및 분화능 분석
    성체줄기세포의 고밀도 응집(micromass formation)
    성체줄기세포에 전기 자극 및 분화능 분석
    통계학적 분석
결 과
    말 지방조직 유래 성체줄기세포의 성상 및 증식능 비교
    말 지방조직 유래 성체줄기세포 세포표면 특이단백질발현 비교
    말 지방조직 유래 성체줄기세포의 중배엽세포 분화능비교
    말 지방조직 유래 성체줄기세포의 마이크로매스 형성및 전기 자극
고 찰
REFERENCES
저자
  • Na-Yeon Gu(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Da-Un Jeong(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Jeong Su Byeon(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Jae-Young Song(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Bang-Hun Hyun(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Jienny Lee(Viral Disease Research Division, Animal and Plant Quarantine Agency) Corresponding Author