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Molecular epidemiological study on a human patient of Brucella melitensis infection occurred in Korea KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Brucellosis is the most common zoonosis worldwide, which is caused by Brucella spp. In humans, it can be mainly occurred by direct contact with infected animals or consumption of contaminated dairy products. This study focused on human brucellosis caused by B. melitensis discovered from Chinese worker in Korea in 2015. We investigated molecular epidemiological evidence to find the infection source. We first performed several PCR methods including 16S rRNA PCR, multiplex PCR and real-time PCR to identify Brucella species. We also conducted MLVA typing for epidemiological trace-back analysis. The isolate from the patient was confirmed to B. melitensis through Brucella-specific PCR. In clustering analysis with B. melitensis from foreign countries, this human isolate was correlated with B. melitensis isolates from humans and sheep in China by 99.9% similarity. Thus, we assumed the brucellosis patient has been already infected in China followed by migration to Korea according to molecular epidemiological analysis with history evidence. Moreover, we suggest it needs to take measures to reduce the risk for intercountry transmission of brucellosis due to the influx of infected people from abroad.

목차
Abstract
서 론
재료 및 방법
    실험 대상
    유전자(PCR) 검사
    Real-time PCR 검사
    Multi-locus VNTR assay (MLVA) typing
결 과
    유전자 PCR 결과
    MLVA typing 결과
고 찰
REFERENCES
저자
  • Eun Ji Yum(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency)
  • Jin-Ju Lee(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency) Corresponding Author
  • Min-Kyeong Kim(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency)
  • Eun-Ji Park(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency)
  • So-Ra Sung(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency)
  • Min-Hoe Lee(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency)
  • Soon-Seek Yoon(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency)
  • Moon Her(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency)
  • Jeong-Soo Choi(Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency)