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High-yield production of porcine parvovirus VP2 protein of Korean strain assembled into virus-like particles in a baculovirus expression system KCI 등재

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  • URLhttps://db.koreascholar.com/Article/Detail/388337
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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.

목차
Abstract
REFERENCES
저자
  • Seyeon Park(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Ju-Yeon Lee(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Jae Young Song(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Soo-dong Cho(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Jienny Lee(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Bang-hun Hyun(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • In-Ohk Ouh(Viral Disease Research Division, Animal and Plant Quarantine Agency/College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) Corresponding Author