Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.

• Seyeon Park(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Ju-Yeon Lee(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Jae Young Song(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Soo-dong Cho(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Jienny Lee(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Bang-hun Hyun(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• In-Ohk Ouh(Viral Disease Research Division, Animal and Plant Quarantine Agency/College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) Corresponding Author