Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprot ein) in regulating fecal malodor and feed efficiency in mice. We found that UDN glycoprotein (200 μg/ml) has an inhibitory effect on the cell death induced by an ecotoxicological endocrine disrupting chemical, bisphenol A, in colon epithelial HT-29 cells. UDN glycoprotein did not show significant differences regarding the weight of ecotoxicity-related organs (liver, heart, kidneys, and spleen) and the levels of serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and lactate dehydrogenase in mice for 2 weeks, compared to the control. Additionally, UDN glycoprotein reduced the levels of hydrogen sulfide and ammonia as markers of fecal malodor in mice. Interestingly, UDN glycoprotein can improve the mouse feed efficiency. In conclusion, our data indicate that anti-ecotoxicological UDN glycoprotein has the ability to increase the feed efficiency and reduce the fecal malodor by maintaining the viability of colonic epithelial cells in mice.

Abstract
1. 서 론
2. 재료 및 방법
2.1. 시약
    2.2. 느릅 당단백질 분리 및 정제
    2.3. 세포배양 및 MTT 분석
    2.4. 사료 효율 및 장기 독성 측정
    2.5. GOT, GPT 및 LDH 측정
    2.6. H2S 및 NH3 농도 측정
    2.7. 통계처리
3. 결과 및 고찰
    3.1. 느릅 당단백질의 장 세포 보호효과 및 환경독성물질이 유도하는 세포사멸 억제효과
    3.2. 느릅 당단백질이 마우스 장기 및 혈액 독성에 미치는 영향
    3.3. 느릅 당단백질이 마우스 사료 효율에 미치는 영향
    3.4. 느릅 당단백질이 마우스 분내 악취저감에 미치는효과
4. 결 론
REFERENCES

• 김도완(대구한의대학교 제약공학과) | Do-Wan Kim (Department of Pharmaceutical Engineering, Daegu Haany University)
• 박문기(대구한의대학교 제약공학과) | Moon-Ki Park (Department of Pharmaceutical Engineering, Daegu Haany University) Corresponding author
• 이세중(대구한의대학교 제약공학과) | Sei-Jung Lee (Department of Pharmaceutical Engineering, Daegu Haany University) Corresponding author