축산 폐수는 고농도의 영양염류와 중금속을 함유하고 있어, 배출될 때 수질을 악화시킨다. 기존 처리 기술과 비교하여 bioremediation은 축산 폐수 처리에 유능하다. 특히, 미세조류는 오염물질 제거에 잠재력을 가지고 있다. 본 연구에서는 Ankistrodesmus bibraianus를 이용하여 축산 폐수 내 영양염류 (질소 (N), 인 (P))와 중금속 (구리 (Cu), 아연 (Zn))의 제거 가능성을 평가하고, A. bibraianus의 최적배양조건을 확립하였다. 연구결과, 최적 배양조건은 28°C, pH 7, 광주기는 14 : 10 h로 설정되었다. N과 P의 단일 처리구 (500, 1,000, 5,000, 10,000 mg L-1)에서 제거효율은 각각 22.9~80.6%와 11.9~50.0%였다. 또한, N과 P의 복합 처리구에서 제거효율은 각각 16.4~58.3%와 7.80~49.8%였다. Cu와 Zn의 단일 처리구 (10, 30, 50 mg L-1)에서 제거효율은 각각 15.5~81.5%와 6.28~34.3%였다. 유사하게, Cu와 Zn의 복합 처리구에서 제거효율은 각각 16.7~74.5%와 5.58~27.5%였다. 또한 영양염류 (N 및 P)와 중금속 (Cu 및 Zn)의 성장 및 제거효율을 축산 폐수에 적용할 수 있음을 나타냈다. 본 연구의 결과에 따르면 A. bibraianus는 축산 폐수 내 영양염류와 중금속 제거에 이용할 수 있을 것으로 사료된다.

Since swine wastewater contains high concentrations of nutrients and heavy metals, it deteriorates water quality when discharged. Compared to conventional methods, bioremediation can be a promising method for its treatment. Specifically, microalgae have the potential to remove these pollutants. In this study, the removal of nutrients (nitrogen (N) and phosphorus (P)) and heavy metals (copper (Cu) and zinc (Zn)) from swine wastewater by Ankistrodesmus bibraianus was evaluated and the organism’s optimal growth conditions were investigated. The optimal growth conditions were established at 28°C, pH 7, and light and dark cycles of 14:10 h. The removal efficiencies of N and P by a single treatment (500, 1,000, 5,000, and 10,000 mg L-1) ranged from 22.9 to 80.6% and from 11.9 to 50.0%, respectively. The removal efficiencies of N and P in the binary treatments ranged from 16.4 to 58.3% and from 7.80 to 49.8%, respectively. The removal efficiencies of Cu and Zn by a single treatment (10, 30, and 50 mg L-1) ranged from 15.5 to 81.5% and from 6.28 to 34.3%, respectively. Similarly, the removal efficiencies of Cu and Zn in the binary treatments ranged from 16.7 to 74.5% and from 5.58 to 27.5%, respectively. In addition, the study showed the optimal growth conditions for microalgae and the removal efficiency of nutrients (N and P) and heavy metals (Cu and Zn), which could be applied to swine wastewater. Based on the results in this study, it appears that Ankistrodesmus bibraianus could be used for the removal of nutrients and heavy metals present in swine wastewater.

Abstract
서 론
재료 및 방법
    1. 미세조류
    2. 최적 배양조건
    3. 합성 폐수
    4. 축산 폐수
    5. 분석방법
    6. 통계분석
결과 및 고찰
    1. 최적 배양조건
    2. 영양염류 (N, P)의 합성 폐수에서 Ankistrodesmus bibraianus의 성장 및 제거효율
    3. 중금속 (Cu, Zn)의 합성 폐수에서 Ankistrodesmus bibraianus의 성장 및 제거효율
    4. 실제 축산 폐수에서 Ankistrodesmus bibraianus 의 성장 및 제거효율
적 요
REFERENCES

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• 박영민(충북대학교 농업생명환경대학 환경생명화학과) | Young-Min Park (Department of Environmental & Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
• 이예은(충북대학교 농업생명환경대학 환경생명화학과) | Ye-Eun Lee (Department of Environmental & Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
• 김덕원(충북대학교 농업생명환경대학 환경생명화학과) | Deok-Won Kim (Department of Environmental & Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
• 박지수(충북대학교 농업생명환경대학 환경생명화학과) | Ji-Su Park (Department of Environmental & Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
• 오은지(충북대학교 농업생명환경대학 환경생명화학과) | Eun-Ji Oh (Department of Environmental & Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
• 유진(경기연구원 생태환경연구실) | Jin Yoo (Department of Ecology & Environment, Gyeonggi Research Institute)
• 정근욱(충북대학교 농업생명환경대학 환경생명화학과) | Keun-Yook Chung (Department of Environmental & Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National University) Corresponding author